General Cell Based Assays
TOPOGEN INC DNA Gyrase Drug Screening Kit
This drug screening Kit is based on gyrase supercoiling action on a relaxed plasmid DNA substrate provided (pHOT1, which is a derivative of pBR322) and roughly 2.7 KB. One unit of gyrase will supercoil 200-500 ng in 1 hr at 37oC. The enzyme introduces negative supercoils into a relaxed pHOT1 plasmid substrate via a sign inversion model. An energy co-factor (ATP) is required for the complete supercoiling reaction. Non-EB gels are ideal for detecting gyrase activity as attested by the excellent resolution between supercoiled and relaxed DNA forms. It is designed to identify IFP and CIC (poisons and inhibitors, respectively). A typical IFP (e.g., a fluoroquinolone) will induce double strand DNA breaks while a prototypical CIC will simply block the enyzme from acting on relaxed DNA (novobiocin, some intercalators for example). Thus, an IFP (ciprofloxacin) will result in formation of a linear DNA product while a CIC will block supercoiling activity.
Eurofins Discoverx PathHunter Human Apelin (APJ Receptor) β-Arrestin-1 Cell-Based Assay Kit (CHO-K1)
The PathHunter Human Apelin (APJ Receptor) β-Arrestin-1 Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure AGTRL1 (GPCR) activity via recruitment of β-Arrestin-1. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Creatinine Assay Kit
Creatinine Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
IPHASE PHARMA SERVICES LLC 5α-Reductase(SRD5A1) Inhibition Kit,200 test (Micropore)
Mainly used in 5α-Reductase(SRD5A2) Inhibition study. It's recommended to purchase the complete reagent kit.
Abcam DPPH Antioxidant Assay Kit (Colorimetric).
DPPH Antioxidant Assay Kit (Colorimetric).
The product is subject to the following: Abcam Restricted Use Statement
Abcam Cell Tracking Dye Kit - Orange - Cytopainter
Cell Tracking Dye Kit - Orange - Cytopainter
The product is subject to the following: Abcam Restricted Use Statement
Abcam Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric).
Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric).
The product is subject to the following: Abcam Restricted Use Statement
Abcam Cell Tracking Dye Kit - Green - Cytopainter
Cell Tracking Dye Kit - Green - Cytopainter
The product is subject to the following: Abcam Restricted Use Statement
ILEX LIFE SCIENCES LLC QuickZyme Hydroxyproline Assay Kit Colorimetric Method 1/EA
QuickZyme Hydroxyproline Assay Kit 2 x 96 Assays (includes 2x 96-well plates) The QuickZyme Hydroxyproline Assay Kit enables quantitative collagen analysis in all types of tissues in a fast and simple 96-well plate format This colorimetric assay does not need the drying step following acid hydrolysis of collagen for which often special equipment is needed This assay is the ONLY hydroxyproline-based assay that does not need this laborious step resulting in a fast sensitive and easy assay NOTE This kit does not contain tubes or a protocol for collagen hydrolysis If you need these items please order the QuickZyme Total Collagen Assay Application conditioned media cell extracts tissues tissue extracts Sensitivity 2 4 M (0 3 g/ml) Assay-time 2 hours QuickZyme Hydroxyproline Assay can be paired with QuickZyme Total Protein Assay to allow the expression of data as hydroxyproline quantity per total amount of protein
STEMCELL Technologies Phalloidin iFluorTM 555
Fluorescent dye conjugates for detection of F-actin in cell culture experiments - 300 Tests
STEMCELL Technologies Wheat Germ Agglutinin WGA i
Fluorescent dye conjugates for staining cell membranes of gram-positive bacteria, mammalian cells, and yeast bud scars - 1000 Ug
Molecular Devices FLIPR Calcium 4 Kit, No-Wash, Fluorescence, 10 Plates
Molecular Devices FLIPR Calcium 4 Assay Kit Explorer provides a no-wash, fluorescence-based method for detecting intracellular calcium changes with improved signal-to-noise using a novel masking dye.
- Designed for no-wash fluorescence calcium assays.
- Includes enough reagent for 10 plates in the Explorer configuration.
- Optimized for small calcium responses with reduced background.
- Compatible with 96-well, 384-well, and 1536-well plate formats.
- Supplied as reagent vials and a dilution/assay buffer to prepare plates.